Day, Alison M., Cove, Jonathan H. and Phillips-Jones, Mary (2003) Cytolysin gene expression in Enterococcus faecalis is regulated in response to aerobiosis conditions. Mol. Genet. Genom., 269 (1). pp. 31-39.
Full text not available from this repository.
Official URL: http://link.springer.com/article/10.1007/s00438-00...
Here we investigate the expression of cylLL and cylLS, the genes that encode the structural subunits of the cytolysin/haemolysin of Enterococcus faecalis, in response to aerobiosis conditions. Haemolysis assays of E. faecalis strains cultured under aerobic and anaerobic conditions revealed three different haemolytic phenotypes, one of which exhibited greater haemolysis under anaerobic conditions than under aerobic conditions, and was shown to be associated with the presence of the cyl genes. Reporter gene studies revealed that cylLLLS promoter activity was significantly greater (up to 8.6- fold) under anaerobic compared to aerobic conditions throughout batch growth, demonstrating that these genes are regulated in response to the degree of aerobiosis. Band shift assays confirmed the binding of a protein factor to the region between 202 and 37 bp upstream of the cylLL start codon, and a higher level of binding was observed with anaerobically derived cellfree extracts than with extracts of aerobically grown cells. This is the first report of an oxygen-regulated virulence factor in E. faecalis (that is distinct from the quorum-sensing regulatory system reported previously), and may be of in vivo relevance for the bacterium in biofilms and other environments characterised by oxygen gradients.
|Subjects:||B - Subjects allied to medicine > B121 - Clinical physiology|
C - Biological sciences > C500 - Microbiology
|Schools:||Faculty of Clinical & Biomedical Sciences > School of Pharmacy and Biomedical Sciences|
|Deposited By:||Mary Phillips-Jones|
|Deposited On:||20 Aug 2015 09:39|
|Last Modified:||17 May 2016 12:54|
Downloads per month over past year
Downloads for past 30 days
Repository Staff Only: item control page