Saidijam, Massoud, Psakis, Georgios, Clough, Joanna L., Mueller, Johan, Suzuki, Shun'ichi, Hoyle, Christopher J., Palmer, Sarah L., Morrison, Scott M., Pos, K., Essenberg, Richard C., Maiden, Martin C.J., Abu-bakr, Atif, Baumberg, Simon G., Stark, Michael J., Ward, Alison, O'Reilley, John, Rutherford, Nicholas G., Phillips-Jones, Mary and Henderson, Peter J.F. (2003) Collection and characterisation of bacterial membrane proteins. FEBS Letters, 555 (1). pp. 170-175.
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
A general strategy for the amplified expression in Escherichia coli of membrane transport and receptor proteins from other bacteria is described. As an illustration we report the cloning of the putative α-ketoglutarate membrane transport gene from the genome of Helicobacter pylori, overexpression of the protein tagged with RGS(His)6 at the C-terminus, and its purification in mg quantities. The retention of structural and functional integrity was verified by circular dichroism spectroscopy and reconstitution of transport activity. This strategy for overexpression and purification is extended to additional membrane proteins from H. pylori and from other bacteria.
|Subjects:||Physical sciences > Chemistry|
B - Subjects allied to medicine > B121 - Clinical physiology
C - Biological sciences > C500 - Microbiology
B - Subjects allied to medicine > B230 - Pharmacy
|Schools:||Faculty of Clinical & Biomedical Sciences > School of Pharmacy and Biomedical Sciences|
|Deposited By:||Mary Phillips-Jones|
|Deposited On:||20 Aug 2015 09:44|
|Last Modified:||17 May 2016 12:54|
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