Dennison, Sarah Rachel, Harris, Frederick, Bhatt, Tailap, Singh, Jaipaul and Phoenix, David Andrew (2009) The effect of C-terminal amidation on the efficacy and selectivity of antimicrobial and anticancer peptides. Molecular and Cellular Biochemistry, 332 (1-2). pp. 43-50. ISSN 0300-8177
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Official URL: http://dx.doi.org/10.1007/s11010-009-0172-8
Cationic defence peptides show high therapeutic potential as antimicrobial and anticancer agents. Some of these peptides carry a C-terminal amide moiety which has been shown to be required for antimicrobial activity. However, whether this is a general requirement or whether C-terminal amidation is required for the anticancer activity of defence peptides is unclear. In response, this study analyses the toxicity of a series of C-terminally amidated defence peptides and their non-amidated isoforms to normal fibroblast cells, a variety of tumour cells and bacterial cells. The toxicities of these peptides to microbial and cancer cells were generally <200 μM. Peptides were either unaffected by C-terminal amidation or showed up to 10-fold decreases or increases in efficacy. However, these peptides all showed toxicity to normal fibroblast cells with levels (generally <150 μM) that were comparable to those of their antimicrobial and anticancer activities. In contrast to previous claims which have been based on analysis of single amidation events, the results of this study clearly show that the C-terminal amidation of defence peptides has a variable effect on their antimicrobial and anticancer efficacy and no clear effect on their selectivity for these cell types.
|Additional Information:||This eprint record has not been checked by the Author.|
|Uncontrolled Keywords (separate with ;):||Antimicrobial peptide ; Anticancer peptide ; Tumour cell lines ; C-terminally amidated|
|Subjects:||R Medicine > RS Pharmacy and materia medica|
|Schools:||College of Science and Technology > School of Computing Engineering & Physical Sciences|
|Deposited By:||Helen Cooper|
|Deposited On:||05 Aug 2010 17:08|
|Last Modified:||27 Jun 2012 13:57|
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