Paulin, Sarah, Jamshad, Mohammed, Dafforn, Timothy R, Garcia-Lara, Jorge, Foster, Simon J, Galley, Nicola F, Roper, David I, Rosado, Helena and Taylor, Peter W (2014) Surfactant-free purification of membrane protein complexes from bacteria: application to the staphylococcal penicillin-binding protein complex PBP2/PBP2a. Nanotechnology, 25 (28). p. 285101. ISSN 0957-4484
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Official URL: http://dx.doi.org/10.1088/0957-4484/25/28/285101
Surfactant-mediated removal of proteins from biomembranes invariably results in partial or complete loss of function and disassembly of multi-protein complexes. We determined the capacity of styrene-co-maleic acid (SMA) co-polymer to remove components of the cell division machinery from the membrane of drug-resistant staphylococcal cells. SMA-lipid nanoparticles solubilized FtsZ-PBP2-PBP2a complexes from intact cells, demonstrating the close physical proximity of these proteins within the lipid bilayer. Exposure of bacteria to (-)-epicatechin gallate, a polyphenolic agent that abolishes β-lactam resistance in staphylococci, disrupted the association between PBP2 and PBP2a. Thus, SMA purification provides a means to remove native integral membrane protein assemblages with minimal physical disruption and shows promise as a tool for the interrogation of molecular aspects of bacterial membrane protein structure and function.
|Uncontrolled Keywords (separate with ;):||Staphylococcus aureus; poly(styrene-co-maleic acid); lipid nanoparticles; antibiotic resistance; immunoaffinity chromatography|
|Subjects:||C - Biological sciences > C500 - Microbiology|
|Schools:||Faculty of Clinical & Biomedical Sciences > School of Medicine|
|Deposited By:||Helen Cooper|
|Deposited On:||14 Jan 2016 15:54|
|Last Modified:||20 Oct 2016 22:52|
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