Bueno, Lígia, El-Sharif, Hazim F., Salles, Maiara O., Boehm, Ryan D., Narayan, Roger J., Paixão, Thiago R.L.C. and Reddy, Subrayal M (2014) MIP-based electrochemical protein profiling. Sensors and Actuators B: Chemical, 204 . pp. 88-95. ISSN 09254005
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Official URL: http://dx.doi.org/10.1016/j.snb.2014.07.100
We present the development of an electrochemical biosensor based on modiﬁed glassy carbon (GC) electrodes using hydrogel-based molecularly imprinted polymers (MIPs) has been fabricated for protein detection. The coupling of pattern recognition techniques via principal component analysis (PCA) has resulted in unique protein ﬁngerprints for corresponding protein templates, allowing for MIP-based protein proﬁling. Polyacrylamide MIPs for memory imprinting of bovine haemoglobin (BHb), equine myoglobin (EMb), cytochrome C (Cyt C), and bovine serum albumin (BSA), alongside a non-imprinted polymer (NIP) control, were spectrophotometrically, and electrochemically characterised using modiﬁed GC electrodes. Rebinding capacities (Q) were revealed to be higher for larger proteins (BHb and BSA, Q ≈ 4.5) while (EMb and Cyt C, Q ≈ 2.5). Electrochemical results show that due to the selective nature of MIPs, protein arrival at the electrode via diffusion is delayed, in comparison to a NIP, by attractive selective interactions with exposed MIP cavities. However, at lower concentrations such discriminations are difﬁcult due to low levels of MIP rebinding. PCA loading plots revealed 5 variables responsible for the separation of the proteins; Ep, Ip, E1/2 , Iat −0.8 V, �Idecay peak current to −0.8 V. Statistical symmetric measures of agreement using Cohen’s kappa coefﬁcient (K) were revealed to be 63% for bare GC, 96% for NIP and 100% for MIP. Therefore, our results show that with the use of PCA such discriminations are achievable, also with the advantage of faster detection rates. The possibilities for this MIP technology once fully developed are vast, including uses in bio-sample clean-up or selective extraction, replacement of biological antibodies in immunoassays, as well as biosensors for medicine, food and the environment.
|Subjects:||Physical sciences > Chemistry|
|Schools:||Faculty of Science and Technology > School of Physical Sciences and Computing|
|Deposited By:||Nuala Jones|
|Deposited On:||16 Feb 2016 17:58|
|Last Modified:||20 Oct 2016 15:39|
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