Earthworm experimentation requires a source of supply. This may come from field-collection, purchase from a supplier, or from breeding of stock. The mode of obtaining the animals may be determined by the type of experimentation, but must not compromise the experiment. Typical collection methods employ digging and hand-sorting of soil, addition of a vermifuge, application of an electrical current to the soil or combinations thereof. Each collection method has advantages and may target particular groups of earthworms more successfully than others. Rearing earthworms in the laboratory may be viewed as difficult but if control of factors such as soil type, moisture, temperature, food supply and stocking density are in place, it can be straightforward. Culture design will be determined by the experimental objectives.