Evaluation of acrylamide-based molecularly imprinted polymer thin-sheets for specific protein capture - a myoglobin model

Sullivan, Mark Vincent, Dennison, Sarah Rachel orcid iconORCID: 0000-0003-4863-9607, Hayes, Joseph orcid iconORCID: 0000-0002-7745-9616 and Reddy, Subrayal M orcid iconORCID: 0000-0002-7362-184X (2021) Evaluation of acrylamide-based molecularly imprinted polymer thin-sheets for specific protein capture - a myoglobin model. Biomedical Physics and Engineering Express .

[thumbnail of Version of Record]
Preview
PDF (Version of Record) - Published Version
Available under License Creative Commons Attribution.

1MB

Official URL: https://doi.org/10.1088/2057-1976/ac0991

Abstract

We evaluate a series of thin-sheet hydrogel molecularly imprinted polymers (MIPs), using a family of acrylamide-based monomers, selective for the target protein myoglobin (Mb). The simple production of the thin-sheet MIP offers an alternative biorecognition surface that is robust, stable and uniform, and has the potential to be adapted for biosensor applications. The MIP containing the functional monomer N-hydroxymethylacrylamide (NHMAm), produced optimal specific rebinding of the target protein (Mb) with 84.9 % (± 0.7) rebinding and imprinting and selectivity factors of 1.41 and 1.55, respectively. The least optimal performing MIP contained the functional monomer N,Ndimethylacrylamide (DMAm) with 67.5 % (± 0.7) rebinding and imprinting and selectivity factors of 1.11 and 1.32, respectively. Hydrogen bonding effects, within a protein-MIP complex, were investigated using computational methods and Fourier transform infrared (FTIR) spectroscopy. The quantum mechanical calculations predictions of a red shift of the monomer carbonyl peak is borneout within FTIR spectra, with three of the MIPs, acrylamide, N-(hydroxymethyl) acrylamide, and N-(hydroxyethyl) acrylamide, showing peak downshifts of 4, 11, and 8 cm-1, respectively.


Repository Staff Only: item control page