Henderson, A, Lee, C, Mistry, V, Thomas , M and Iyengar, Arati
Reliable and robust molecular sexing of the hen harrier (Circus cyaneus) using PCR-RFLP of the CHD1 gene.
Journal of Forensic Sciences, -
ISSN 0022-1198; 1556-4029
Official URL: http://dx.doi.org/10.1111/1556-4029.12030
The hen harrier (Circus cyaneus) is a bird of prey that is persecuted in the United Kingdom, and there is a need for a DNA-based individual identification and sexing system for the use in forensic investigations. This study reports a new set of PCR primers for the chromo-helicase-DNA-binding protein 1 gene, which allows sexing using PCR-RFLP. Instead of exonic primers that amplify across a large intron, this set consists of a primer within the intron, enabling reduction in amplicon sizes from 356 to 212 bp and 565 to 219 bp in W and Z chromosomes. DNA degradation and dilution experiments demonstrate that this set is significantly more robust than one that amplifies across the intron, and sequencing of the intronic primer-binding region across several individuals shows that it is highly conserved. While our objective is to incorporate this primer set into an STR-based individualization kit, it may in the meantime prove useful in forensic or conservation studies.
|Uncontrolled Keywords (separate with ;):|| forensic science;
bird of prey;
chromo-helicase-DNA-binding protein 1|
|Subjects:||Q Science > QK Botany|
|Schools:||School of Forensic & Investigative Sciences|
|Deposited On:||29 Mar 2012 07:59|
|Last Modified:||06 Mar 2013 17:29|
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