Baker, Matthew James, Zheng, Leiliang, Winograd, Nicholas, Lockyer, Nicholas P. and Vickerman, John C. (2008) Mass Spectral Imaging of Glycophospholipids, Cholesterol, and Glycophorin A in Model Cell Membranes. Langmuir, 24 (20). pp. 11803-11810. ISSN 0743-7463
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Official URL: http://dx.doi.org/10.1021/la802582f
of flight secondary ion mass spectrometry (ToF-SIMS) and the Langmuir-Blodgett (LB) technique have been used to create and analyze reproducible membrane mimics of the inner and outer leaflets of a cellular membrane to investigate lipid-protein and lipid-lipid interactions. Films composed of phospholipids, cholesterol and an integral membrane protein were utilized. The results show the outer membrane leaflet mimic (DPPC/cholesterol/ glycophorin A LB film) consisting of a single homogeneous phase whereas the inner membrane leaflet mimic (DPPE/cholesterol/glycophorin A LB film) displays heterogeneity in the form of two separate phases. A DPPE/cholesterol phase and a glycophorin A phase. This points to differences in membrane domain formation based upon the different chemical composition of the leaflets of a cell membrane. The reliability of the measurements was enhanced by establishing the influence of the matrix effect upon the measurement and by utlilizing PCA to enhance the contrast of the images.
|Uncontrolled Keywords (separate with ;):||Cellular membranes; Chemical compositions; Glycophorin A; Inner membranes; Integral membrane proteins; Langmuir-blodgett; LB films; Lipid interactions; Mass spectral; Matrix effects; Membrane domains; Outer membranes; Time-of-flight secondary ion mass spectrometries|
|Subjects:||B - Subjects allied to medicine > B230 - Pharmacy|
|Schools:||Faculty of Science and Technology > School of Forensic and Applied Sciences|
|Deposited By:||Simone Finley|
|Deposited On:||21 Feb 2013 18:03|
|Last Modified:||15 Mar 2017 10:44|
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