Ogden, Samantha (2003) The optimisation of protocols for the extraction of nuclear DNA from animal bone forPCR. Doctoral thesis, University of Central Lancashire.
PDF (Thesis Document)
- Accepted Version
Available under License Creative Commons Attribution Non-commercial Share Alike.
The general aims of this research were to find an effective method for extracting DNA from bone and to use this methodology to create Randomly Amplified Polymorphic DNA- Polymerase Chain Reaction (RAPD-PCR) profiles of different species. A modified Chelex method was selected as the preferred extraction procedure and RAPD-PCR profiles were created for several species using a number of different primers. Individuality between closely related species was also achieved. This research could go on to help in the archaeological field with identifj'ing
unknown bone fragments.
|Item Type:||Thesis (Doctoral)|
|Uncontrolled Keywords (separate with ;):||multifunctional system; Bone composition; endocrinology; endocrinology|
|Schools:||Faculty of Science and Technology > School of Forensic and Applied Sciences|
|Deposited By:||Paul Harrison|
|Deposited On:||19 Dec 2013 16:44|
|Last Modified:||10 Feb 2017 12:42|
Downloads per month over past year
Downloads for past 30 days
Repository Staff Only: item control page