Automating the application of smart materials for protein crystallization

Khurshid, S., Govada, L., El-Sharif, H.F., Reddy, Subrayal M orcid iconORCID: 0000-0002-7362-184X and Chayen, N.E. (2015) Automating the application of smart materials for protein crystallization. Acta Crystallographica Section D: Biological Crystallography, 71 . pp. 534-540. ISSN 1399-0047

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Official URL: http://dx.doi.org/10.1107/S1399004714027643

Abstract

The fabrication and validation of the first semi-liquid nonprotein nucleating agent to be administered automatically to crystallization trials is reported. This research builds upon prior demonstration of the suitability of molecularly imprinted polymers (MIPs; known as 'smart materials') for inducing protein crystal growth. Modified MIPs of altered texture suitable for high-throughput trials are demonstrated to improve crystal quality and to increase the probability of success when screening for suitable crystallization conditions. The application of these materials is simple, time-efficient and will provide a potent tool for structural biologists embarking on crystallization trials. © 2015, IUCR. All rights reserved.


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