Equation to Line the Borders of the Folding–Unfolding Transition Diagram of Lysozyme

Mohammad, Mohammad Amin, Grimsey, Ian M. and Forbes, Robert Thomas orcid iconORCID: 0000-0003-3521-4386 (2016) Equation to Line the Borders of the Folding–Unfolding Transition Diagram of Lysozyme. Journal of Physical Chemistry B, 120 (28). pp. 6911-6916. ISSN 1520-6106

[thumbnail of Author Accepted Manuscript]
Preview
PDF (Author Accepted Manuscript) - Accepted Version
Available under License Creative Commons Attribution Non-commercial.

452kB

Official URL: http://dx.doi.org/10.1021/acs.jpcb.6b01317

Abstract

It is important for the formulators of biopharmaceuticals to predict the folding-unfolding transition of proteins. This enables them to process proteins under predetermined conditions, without denaturation. Depending on the apparent denaturation temperature (Tm) of lysozyme, we have derived an equation describing its folding-unfolding transition diagram. According to the water content and temperature, this diagram was divided into three different areas, namely, the area of the water-folded lysozyme phase, the area of the water-folded lysozyme phase and the bulk water phase, and the area of the denatured lysozyme phase. The water content controlled the appearance and intensity of the Raman band at ∼1787 cm(-1) when lysozyme powders were thermally denatured at temperatures higher than Tm.


Repository Staff Only: item control page