A comparison of the effects of an Ascophyllum nodosum ethanol extract and its molecular weight fractions on the inflammatory immune gene expression in-vitro and ex-vivo

Abstract

The anti-inflammatory bioactivities of three crude extracts of Ascophyllum nodosum (cold water, hot water and 80% ethanol extract (EE)) were initially evaluated. Following from this, the immune modulatory efficacy of EE and its MW fractions (< 3.5, 3.5–100, > 100 kDa) were compared. In TNF-α challenged Caco-2 cells, the crude extracts reduced IL-8 production (P < 0.001), while the EE caused a > 2 fold down-regulation of; cytokines (IL8, TNFA, IL1B, IL18 and CSF1) chemokines (CXCL10 and CCL5), components of NF-κB pathway (NFKB2 and IKBKB), and inflammatory mediators (PTGS2 and MIF) genes. In the porcine colonic tissue ex-vivo challenged with lipopolysaccharide, EE and its MW fractions down-regulated the immune related targets LYZ, IL8, PTGS2, TLR6, CXCL10, IL6, CXCL11, ICAM, NFKB1 and CXCL2. It was concluded that while the MW fractionation potentially generated compounds displaying distinct immune modulatory functions, the crude EE exhibited the most potent immunomodulatory bioactivity.