Nile Red staining of neutral lipids in yeast

Rostron, Kerry and Lawrence, Clare Louise orcid iconORCID: 0000-0003-0170-0079 (2017) Nile Red staining of neutral lipids in yeast. In: Histochemistry of Single Molecules. Methods in Molecular Biology, 1560 . Springer, pp. 219-229. ISBN 978-1-4939-6787-2

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Determination of cellular neutral lipid levels in yeast is important for both the biotechnology industry and biomedical research. However, many of the currently available methods are labour intensive and time consuming. Here we describe a rapid and repeatable method for the detection of neutral lipids, which can be utilised in both oleaginous and non-oleaginous yeast species. The method utilises the fluorescent dye, Nile red, which enables neutral lipid levels to either be visualised via microscopy or quantified using a 96 well plate assay.

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