Low pre-exercise muscle glycogen availability offsets the effect of post-exercise cold water immersion in augmenting PGC-1α gene expression

Allan, Robert orcid iconORCID: 0000-0002-9021-8737, Sharples, AP, Cocks, M, Drust, B, Dutton, J, Dugdale, HF, Mawhhinney, C, Clucas, A, Hawkins, W et al (2019) Low pre-exercise muscle glycogen availability offsets the effect of post-exercise cold water immersion in augmenting PGC-1α gene expression. Physiological Reports, 7 (11).

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Official URL: https://doi.org/10.14814/phy2.14082

Abstract

We assessed the effects of post-exercise cold-water immersion (CWI) in modulating PGC-1α expression in response to exercise commenced with low muscle glycogen availability. In a randomized repeated-measures design, nine recreationally active males completed an acute two-legged high-intensity cycling protocol (8 x 5 min at 82.5% peak power output) followed by 10 minutes of two-legged post-exercise CWI (8°C) or control conditions (CON). During each trial, one limb commenced exercise with low (LOW: <300 mmol.kg-1 dw) or very low (VLOW: <150 mmol.kg-1 dw) pre-exercise glycogen concentration, achieved via completion of a one-legged glycogen depletion protocol undertaken the evening prior. Exercise increased (P<0.05) PGC-1α mRNA at 3 h post-exercise. Very low muscle glycogen attenuated the increase in PGC-1α mRNA expression compared with the LOW limbs in both the control (CON VLOW ~3.6-fold vs. CON LOW ~5.6-fold: P = 0.023, ES 1.22 Large) and CWI conditions (CWI VLOW ~2.4-fold vs. CWI LOW ~8.0 fold: P = 0.019, ES 1.43 Large). Furthermore, PGC-1α mRNA expression in the CWI-LOW trial was not significantly different to the CON LOW limb (P = 0.281, ES 0.67 Moderate). Data demonstrate that the previously reported effects of post-exercise CWI on PGC-1α expression (as regulated systemically via β-adrenergic mediated cell signalling) are offset in those conditions in which local stressors (i.e. high-intensity exercise and low muscle glycogen availability) have already sufficiently activated the AMPK- PGC-1α signaling axis. Additionally, data suggest that commencing exercise with very low muscle glycogen availability attenuates PGC-1α signaling.


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