Heparan sulfate disaccharide measurement from biological samples using precolumn derivatization, UPLC-MS and single ion monitoring

Antia, Imeobong orcid iconORCID: 0000-0001-6620-1903, Yagnik, Darshna, Pantoja Munoz, Leonardo, Shah, Ajit and Hills, Frank (2017) Heparan sulfate disaccharide measurement from biological samples using precolumn derivatization, UPLC-MS and single ion monitoring. Analytical Biochemistry, 530 . pp. 17-30. ISSN 0003-2697

[thumbnail of Author Accepted Manuscript]
Preview
PDF (Author Accepted Manuscript) - Accepted Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

976kB

Official URL: https://doi.org/10.1016/j.ab.2017.04.019

Abstract

Glycosaminoglycans are a heterogeneous family of linear polysaccharides comprised of repeating disaccharide subunits that mediate many effects at the cellular level. There is increasing evidence that the nature of these effects is determined by differences in disaccharide composition. However, the determination of GAG disaccharide composition in biological samples remains challenging and time-consuming. We have developed a method that uses derivatization and selected ion recording and RP-UPLCMS resulting in rapid separation and quantification of twelve heparin/heparin sulfate disaccharides from 5 μg GAG. Limits of detection and quantitation were 0.02-0.15 and 0.07-0.31 μg/ml respectively. We have applied this method to the novel analysis of disaccharide levels extracted from heparan sulfate and human cancer cell lines. Heparan sulfate disaccharides extracted from biological samples following actinase and heparinase incubation and derivatized using reductive amination with 2-aminoacridone. Derivatized disaccharides were analyzed used UPLC-MS with single ion monitoring. Eight HS disaccharide subunits were separated and quantified from HS and cell lines in eleven minutes per sample. In all samples the most abundant subunits present were the unsulfated ΔUA-GlcNAc, ΔUA-GlcNAc,6S and ΔUA,2S-GlcNS,6S. There was considerable variation in the proportions and concentrations of disaccharides between different cell lines. Further studies are needed to examine the significance of these differences.


Repository Staff Only: item control page