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Concordance Between the AmpFℓSTR®MiniFiler™ and AmpFℓSTR®Identifiler®PCR Amplification Kits in the Kuwaiti Population

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Alenizi, Mohammad A., Goodwin, William H orcid iconORCID: 0000-0002-3632-3552, Hadi, Sibte, Alenizi, Homod H., Altamar, Khaleda A. and Alsikel, Mona S. (2009) Concordance Between the AmpFℓSTR®MiniFiler™ and AmpFℓSTR®Identifiler®PCR Amplification Kits in the Kuwaiti Population. Journal of Forensic Sciences, 54 (2). pp. 350-352. ISSN 00221198

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Official URL: http://dx.doi.org/10.1111/j.1556-4029.2008.00962.x

Abstract

The AmpFℓSTR® MiniFiler™ polymerase chain reaction amplification kit, developed and supplied by Applied Biosystems, complements the AmpFℓSTR® Identifiler® polymerase chain reaction amplification kit (Applied Biosystems, Warrington, U.K.) by improving the success rate when profiling DNA that is degraded or contains inhibitors. Before applying the MiniFiler™ kit to casework, the profiles from 200 unrelated Kuwaitis were compared to Identifiler® profiles. Concordance was observed for 99.875% (1598 of 1600) of the compared STR loci. The two discordant profiles displayed allelic dropout: one at the D13S317 locus due to nonamplification of allele 10 in the MiniFiler™ profile, and one at the D18S51 locus due to nonamplification of allele 18 in the Identifiler® profile.


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