Nanostructural Studies on Monoelaidin–Water Systems at Low Temperatures

Abstract

In recent years, lipid based nanostructures have increasingly been used as model membranes to study various complex biological processes. For better understanding of such phenomena, it is essential to gain as much information as possible for model lipid structures under physiological conditions. In this paper, we focus on one of such lipids—monoelaidin (ME)—for its polymorphic nanostructures under varying conditions of temperature and water content. In the recent contribution (Soft Matter, 2010, 6, 3191), we have reported the phase diagram of ME above 30 °C and compared with the phase behavior of other lipids including monoolein (MO), monovaccenin (MV), and monolinolein (ML). Remarkable phase behavior of ME, stabilizing three bicontinuous cubic phases, motivates its study at low temperatures. Current studies concentrate on the low-temperature (<30 °C) behavior of ME and subsequent reconstruction of its phase diagram over the entire temperature–water composition space (temperature, 0–76 °C; and water content, 0–70%). The polymorphs found for the monoelaidin–water system include three bicontinuous cubic phases, i.e., Ia3d, Pn3m, and Im3m, and lamellar phases which exhibit two crystalline (Lc1 and Lc0), two gel (Lβ and Lβ*), and a fluid lamellar (Lα) states. The fluid isotropic phase (L2) was observed only for lower hydrations (<20%), whereas hexagonal phase (H2) was not found under studied conditions. Nanostructural parameters of these phases as a function of temperature and water content are presented together with some molecular level calculations. This study might be crucial for perception of the lyotropic phase behavior as well as for designing nanostructural assemblies for potential applications.