Effect of PEG Crystallization on the Self-Assembly of PEG/Peptide Copolymers Containing Amyloid Peptide Fragments

Abstract

The effect of poly(ethylene glycol) PEG crystallization on β-sheet fibril formation is studied for a series of three peptide/PEG conjugates containing fragments modified from the amyloid β peptide, specifically KLVFF, FFKLVFF, and AAKLVFF. These are conjugated to PEG with Mn = 3300 g mol−1. It is found, via small-angle X-ray scattering, X-ray diffraction, atomic force microscopy, and polarized optical microscopy, that PEG crystallinity in dried samples can disturb fibrillization, in particular cross-β amyloid structure formation, for the conjugate containing the weak fibrillizer KLVFF, whereas this is retained for the conjugates containing the stronger fibrillizers AAKLVFF and FFKLVFF. For these two samples, the alignment of peptide fibrils also drives the orientation of the attached PEG chains. Our results highlight the importance of the antagonistic effects of PEG crystallization and peptide fibril formation in PEG/peptide conjugates.