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The optimisation of protocols for the extraction of nuclear DNA from animal bone forPCR.

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Ogden, Samantha (2003) The optimisation of protocols for the extraction of nuclear DNA from animal bone forPCR. Doctoral thesis, University of Central Lancashire.

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Abstract

The general aims of this research were to find an effective method for extracting DNA from bone and to use this methodology to create Randomly Amplified Polymorphic DNA- Polymerase Chain Reaction (RAPD-PCR) profiles of different species. A modified Chelex method was selected as the preferred extraction procedure and RAPD-PCR profiles were created for several species using a number of different primers. Individuality between closely related species was also achieved. This research could go on to help in the archaeological field with identifj'ing
unknown bone fragments.

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