Collection Methods for Touch DNA Direct Amplification

Alketbi, Salem Khalifa orcid iconORCID: 0000-0002-7773-3953 and Goodwin, William H orcid iconORCID: 0000-0002-3632-3552 (2023) Collection Methods for Touch DNA Direct Amplification. Journal of Forensic Legal & Investigative Sciences, 9 (72). pp. 1-3.

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DNA direct amplification from biological samples such as saliva and blood has proven to be successful but can be more challenging from minute samples such as trace DNA. Developing new protocols to collect and process Touch DNA samples can improve DNA direct amplification, therefore a variety of collection methods were investigated for their efficiency to collect Touch DNA for direct amplification using a GlobalFiler™ PCR amplification Kit. Touch Samples were collected from six surfaces to test the sensitivity of the collection method for direct amplification, with the number of alleles observed from the DNA profiles significantly affected by collection type (p < 0.001). Copan microFLOQ swabs (MF) and SceneSafe Fast™ Minitapes (MT) recovered a higher percentage of alleles and were more reliable for direct DNA amplification.

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